Liquid Chromatography with LTQ Orbitrap mass spectrometer
| Description | |
| Entity attached to: | |
| Platform | METABOMER : Mass Spectrometry Core Facility |
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| Type of entity | Equipment |
| Main activity | UHPLC Ultimate 3000 (Dionex). LTQ Orbitrap (Thermo): LTQ: Linear Ion Trap, Orbitrap: Electrostatic Ion Trap, also called the Fourier Transform Orbital Trap (FTMS). The mass spectrometer is coupled to a UHPLC Ultimate 3000 (Dionex) for metabolomic analyzes. The ionization of the sample is of electrospray (ESI) type and can be done in positive and/or negative mode. The mass range can range from 0 to 2000 in m/z ratio, at the LTQ trap (first analyzer) and the orbitrap (second analyzer). This mass spectrometer also has a direct infusion device, offering maximum optimization of the detection conditions. We use Xcalibur (Thermo) software and Worflow4Metabolomics for data processing, spectrum analysis, statistical analysis, open database querying ... This workflow allows to obtain a file regrouping the masses eluted at the same retention time in the same group (pcgroup), any adducts and/or isotopes, to target a particular zone during an analysis in non-targeted mode. The platform has certain standards (amino acids, fatty acids, sterols ...) for targeted analysis. Coupled with the UHPLC, it is used for analyzes (qualitative and/or quantitative) of marine metabolites: seawater, fluids, tissues ... More specifically, the platform analyzes for example collodaries, bacteria, microalgae, dinoflagellates (eg: gymnodinium, brandtodinium and pelagodinium) ... |
| Keywords | Non-Targeted Analysis, Quantification, Liquid Chromatography, Statistical, Metabolome, Fragmentation |
| Labels | Biogenouest Recognised French National Research Infrastructure (IR) EMBRC-France EMBRC-ERIC |
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Location and contact
| Send an e-mail | |
| Adress | Station biologique de Roscoff - Place Georges Teissier |
| Address line 2 | |
| Zip code/Postcode | 29680 |
| Town/city | ROSCOFF |
Description
| Category | Land-based tests and test sites for engineering and ocean observation Analyses, measurements and metrology |
| hasafcfaf18-c675-48af-beca-8bb1ff090072 | |
| Scientific disciplines | Marine biochemistry / Marine biogeochemistry / Marine chemistry Bioinformatics Marine Ecology |
| Strategic areas of action |  Environmental and coastal planning and developmentMarine biological resources |
| Examples of work | |
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Roscoff Marine Station The Roscoff Marine Station is a research and teaching centre for marine biology and ecology. It depends on the CNRS and Sorbonne University. |
